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Q. I have a dual-boot computer, can I convert to FAT32?
A. If you convert your hard drive to FAT32 using Drive Converter, you can no longer use dual boot to run earlier versions of Windows (Windows 95 version 4.00.950, Microsoft Windows NT 3.x, Microsoft Windows NT 4.0, and Microsoft Windows 3.x). However, if you are on a network, earlier versions of Windows can still gain access to your FAT32 hard drive using the network.
Microsoft Windows Platform Development:
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MS-DOSĀ® APIs that rely on intimate knowledge of the file system layout generally fail on FAT32 drives. For example, GetDPB (Int 21h, function 32h), Int 25/26h Absolute Disk Read/Write, and most of the Int 21h, function 440Dh IOCTLs will fail on FAT32 drives.