Yes, once you have transferred the files from the flash drive verify they have transferred successfully. You can then remove the files from the flash drive, but I would recommend backing up all the data on your XP maching regularly. Even a simple copy of all your folders to a larger flash drive would ensure you always had the files you needed even should you have something like a hard drive failure. Just get a flash drive for the sole purpose of backup. There are other backup methods but this is simple.